Stable Production of Antibodies

Stable Production of Antibodies

 
Wild type Antibodies
Compared with monoclonal antibodies derived from polyclonal antibodies and hybridoma, the recombinant antibody has the following advantages: 1) the antibody recognizes single epitope and has repeatability and specificity; 2) the influence of the instability of the hybrid tumor on the expression of the antibody was solved. 3) It can be modified on the sequence of antibodies to meet various needs; 4) mass production; 5) it is easier to control the differences between batches.
 
The technical team of our company has rich experience in stable expression of antibodies in CHO and HEK293FT cells can provide no virus, serum-free suspension culture, antibodies modification, and other special requirements, and provide flexible and quality services for customers. 
 
Glyco-Engineered Antibodies
Studies on the biological significance of ADCC enhancement in the Glyco-Engineered Antibodies have promoted its application in the treatment of diseases, especially the treatment of cancer. The company has successfully developed the lack of fucose CHO cells line, Fut8-/- GS-/-cho cell lines, and successfully expressed and produced various antibodies with these cells. We are committed to flexible business cooperation with customers both at home and abroad, to provide customers with high quality lack of fucose antibodies, promote the research on the mechanism of its enhanced ADCC effect, which is used in the treatment of human diseases.
 
Our advantages
  1. One-stop service: solve all problems for you, from the plasmid construction to the purification of antibodies.
  2. Qualified cell lines: CHO-K1 cell lines purchased from ATCC to ensure the expression of antibody quality.
  3. Time saving: to save time cost for you by choose rapid growth and high efficient transfection cells, fast plasmid construction strategy, antibodies expression analysis techniques, cell culture and purification methods,.
  4. The high expression level: significantly increase the production of antibody expression by the plasmid sequence effect (with strong secretion signal peptide, codon optimization of gene sequences, the small molecular weight of plasmid, , etc.), method of transfection and cell culture, etc.,
  5. The high quality of the antibodies: ensure the activity of antibody, inactivated viruses, reduce the formation of polymer using affinity chromatography, ion exchange chromatography, gel filtration chromatography, thin layer chromatography and dialysis and ultrafiltration method.
  6. High success rate: more than 80 antibodies have been successfully expressed in our mammalian expression system.
  7. Mass production: several gram-level antibodies can be obtained through suspension culture.
  8. Solve the problem of low antibody expression: Make the antibody expression as much as possible by optimizing the gene sequence codon; Select the appropriate signal peptide to secrete the antibody into the cell to reduce the effect on the normal physiological activity of the cell; the gene sequence is mutated without affecting the function of the antibody to reduce the concentration of antibodies and increase the yield by means of bioinformatics strategy.
  9. Antibody modification: Provide you with the most appropriate antibodies by specialized antibody structure analysis and transformation

Antagen Company can product antibody be customized for you in different requirements of antibody, customers can choose different cell lines and different ways of expression, and then select the expression of different size (30 mL-1 L).

Antibody expression process

 
Plasmid construction (1 ~3 weeks)
  • Codon optimization of gene sequence;
  • Physicochemical properties and spatial structure prediction analysis of the antibodies;Selecting the appropriate signal peptide and vector;
  • Plasmid construction;
  • Plasmid extraction
Transfection (0.5 day)
  • Chemical transfection or electrical transfection;
Evaluation of antibody expression in a small amount (2 ~ 3 weeks)
  • Expression analysis of antibody in cell culture: ELISA, Western Blotting, IHC, ICC, Flow Cyt, IP, SDS-PAGE, etc.
  • Antibody purification: using affinity chromatography, ion exchange chromatography, gel filtration chromatography, hydrophobic chromatography, dialysis, ultrafiltration, etc.
  • Antibody identification: SDS-PAGE, physicochemical property analysis, virus content analysis, HCP analysis, mass spectrum analysis, titer analysis, ADCC activity analysis, specific analysis, etc.
Mass antibody expression and purification (2 ~ 3 weeks)
  • Cell transfection and suspension culture;
  • Antibody purification: using affinity chromatography, ion exchange chromatography, gel filtration chromatography, hydrophobic chromatography, dialysis, ultrafiltration, etc.
  • Antibody identification: SDS-PAGE, physicochemical property analysis, virus content analysis, HCP analysis, mass spectrum analysis, titer analysis, specific analysis, etc.
The final delivery (1 ~ 3 days)
  • Antibody and QC data and experimental report;